Bachelet group install Capillary Electrophoresis instrument
February 9, 2016
the Bachelet group have installed and configured a new, state-of-the-art Sciex P/ACE MDQ Capillary Electrophoresis instrument, and already performed high-resolution rounds of aptamer selection on LIVING CELLS (to the best of our knowledge, for the first time) of HEK293 origin (the same ones on which Hana3A [used for hORs] are based and the same ones which will be used for expression of DmelORs). Once we validate binding by flow cytometry we will send to sequencing. We are still working on next-generation sequencing of aptamers, which is challenging due to the existence of common regions flanking each random region.