EXPRESSING CELLS SHIPPED FROM MPG

As part of our task to generate transgenic cell lines expressing functional olfactory receptors, the Hansson group have delivered to the Bachelet group the following plasmids containing DNA for ORs: pcDNA3.1-DmelOR22a (Dmel=D. melanogaster), pcDNA3.1-DmelOR56a, pcDNA3.1-DmelOrco (OR coreceptor), pcDNA3.1(+)RTP1S. In addition they conveyed precise detailed protocols for expressing these receptors in HEK293 cells and protocols for functional calcium imaging in response to DNA/RNA NANOSMELLS